FACILITIES

The laboratory space is located at the McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, University of Pittsburgh and within the Department of Cardiothoracic Surgery. Approximately 73,000 square feet of office, laboratory and conference room space is dedicated to the Institute between two buildings. The Bridgeside Point II building, where the PI’s laboratory is located, houses over 33,000 square feet of McGowan labs, offices and conference rooms that focus on tissue engineering and regenerative medicine. The open floor plan of Bridgeside Point II was specifically designed to facilitate collaboration and aid with intellectual exchange.

The Thoracic Aortic Disease Research Laboratory fully supports tissue processing and cell culture, microscopy, molecular biology and biochemistry specified in this proposal. Special resources include BSL-II biosafety cabinets for human tissue culture, hypoxic chambers, vascular bioreactor systerm, an inverted fluorescent microscope, live-cell stage-top incubation chamber, digital imaging cameras (monochromatic and color), and imaging software. Shared common resources in adjacent laboratory spaces of the McGowan labs including a 4-channel wire myograph (DMT); perfusion myograph (DMT); surgical microscope (ISIS); autoclave, glass washers, drying oven, nanopure water system and ice machine. Special resources within the laboratory space are BSL-IIA biosafety cabinets for human tissue culture and work with viral vectors, an inverted fluorescent microscope, live-cell stage-top incubation chamber, digital imaging cameras (monochromatic and color) and imaging software, a custom cell seeding device and vascular graft bioreactors. Specific equipment in the laboratory include:

  • Inverted Fluorescence Eclipse TE2000-E Microscope with Intensilight System E – Nikon
  • DS-Fi1 5MP Color Camera – Nikon
  • Nikon Advanced Research Workstation with NIS Elements Software – Nikon
  • Compound Microscope – Leitz
  • CO2 Injected Incubator – Thermo Electron Corp. (2)
  • Custom Lumegen™ 6-chamber Vascular Bioreactor (2)-Instron TGT
  • Centrifgue-Sorvall
  • Tissue Culture Hood – Thermo
  • Liquid Nitrogen Dewars (3) – Thermo Electron Corp.
  • -80ฐC Freezer – Thermo Electron Corp.
  • Refrigerator/Freezer – Amana
  • Analytical Scale – Denver Instruments
  • Water bath – Precision Scientific; Hotplate/Stirrer – VWR
  • Microplate reader for absorbance, fluoresence and luminescence- TECAN\
  • MACS Magnetic Bead Cell Isolation Apparatus – Miltenyi Biotec
  • Rocker – Reliable Scientific
  • Vacuum Manifold – Promega
  • Gel Electrophoresis Power Source – Biorad
  • Custom fresh tissue slicing block (in-house machining)

  • Imaging Array CoolSNAP ES2 Monochrome 1394x1040 High Resolution Camera – Photometrics
  • Perfect Focus System-Nikon
  • Live Cell Incubator for time-lapse miscroscopy and tri-gas mixer-Tokai Hit
  • High Temp, O2 Control, CO2 Injector tri-gas Incubator-New Brunswick Scientific (2)
  • Custom-built rotational vacuum cell seeding device-Instron TGT
  • Temperature Regulated Centrifuge – Sorvall
  • Microcentrifuges – Eppendorf (2)
  • Chemical Hood – Hamilton
  • -20ฐC Freezer – Kenmore
  • Accumet pH Meter – Fisher Scientific
  • Isotemp Heating Block – Fisher Scientific
  • Rocker/Incubator – Lab Line
  • gentleMACSTM Dissociator – Miltenyi Biotec
  • Qubitฎ 2.0 Fluorometer- Life Technologies
  • Vortex (3) – Fisher Scientific
  • 2 Horizontal Gel Electrophoresis Systems – Biorad
  • Chemidoc XRS Bioimaging Station- Bio-Rad



Updated 18-Aug-2014