Pitt Study Finds Mechanism for Increased Activity of Oncogene in Head and Neck Cancers
In a recent study led by researchers at the University of Pittsburgh School of Medicine, McGowan Institute for Regenerative Medicine affiliated faculty member Ivet Bahar, PhD, the John K. Vries chair and professor in the Department of Computational & Systems Biology at the University of Pittsburgh and the associate director of the University of Pittsburgh Drug Discovery Institute, and colleagues report the increased activation of a key oncogene in head and neck cancers could be the result of mutation and dysfunction of regulatory proteins that are supposed to keep the gene, which has the potential to cause cancer, in check. The findings, published in The Proceedings of the National Academy of Sciences, suggest a new target for drugs to treat head and neck tumors, as well as other cancers.
Many research teams have found activation and increased signaling of a protein known as Signal Transducer and Activator of Transcription 3 (STAT3) in different kinds of cancers, and it is associated with poor prognosis, said senior author Jennifer Grandis, MD, Distinguished Professor of Otolaryngology, Pitt School of Medicine, and director of the Head and Neck Program at the University of Pittsburgh Cancer Institute (UPCI), partner with UPMC Cancer Center. In adult tissues, STAT3 triggers the production of other proteins that promote the growth and survival of cancer cells.
“Until now, the question of why STAT3 could be hyperactivated has gone unanswered,” Dr. Grandis said. “Our findings reveal a possible mechanism for this abnormal activity, which could help us develop new cancer drugs.”
Noting that gene aberrations in STAT3 itself rarely occurred in head and neck cancers, she and her colleagues looked for mutations in other proteins associated with increased activity of STAT3. To be activated, STAT3 must be phosphorylated, meaning a phosphate group is added to it. Many cancer drugs work by inhibiting enzymes called kinases that encourage this process. The team focused instead on the other side of the biochemical seesaw in which enzymes called phosphatases deactivate proteins by removing phosphates.
To their surprise, they found head and neck tumors with elevated STAT3 were associated with mutations in the PTPR family of phosphatases. When they reproduced the mutations in computational and lab models, they saw that they led to dysfunction of the enzymes.
“Because the phosphatases don’t work properly, phosphate groups don’t get removed from STAT3 appropriately, and it stays activated,” Dr. Grandis explained. “These mutations essentially get rid of the brakes that might otherwise slow or even stop cancer development.”
It might be possible one day to screen tumors for mutations in the PTPR group and then treat them with drugs that inhibit STAT3’s activity, she added.
Abstract (Frequent mutation of receptor protein tyrosine phosphatases provides a mechanism for STAT3 hyperactivation in head and neck cancer. Lui VW, Peyser ND, Ng PK, Hritz J, Zeng Y, Lu Y, Li H, Wang L, Gilbert BR, General IJ, Bahar I, Ju Z, Wang Z, Pendleton KP, Xiao X, Du Y, Vries JK, Hammerman PS, Garraway LA, Mills GB, Johnson DE, Grandis JR. The Proceedings of the National Academies of Science; online 2014 Jan 6.)